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Newcastle disease virus fusion protein gene expression of avian pox virus of the recombinant plasmid

ntheinteractionofliposome, therecombinantvirusrFPVFGFPwasgener-atedviaCO-transfectionofthefowlpoxvirusgenomeandthedeliveryvector, inwhichtandeminsertionofthefusiongenecassetteandloxp-GFP-loxPsequenceintothehomologousarmgeneoffowlpoxvirus, intochickembryofibroblastcel1. Aftersecondtransfection, theGFPgeneinrecombinantgenomewasremovedautomaticallybysitespecificrecombinationofCre-loxPsystemandthentherFPVFwasgainedonlycontainingfusiongeneofNDV. TherFPVFrecombinantcouldmuhiplicatestablyinCEFandthefusionproteinexpressionproductgotimmunogenicabilitytopromotethechickentoproduceneutralizingantibodyagainstNDV. Keywords: Fowpoxvirus; Newcastlediseasevirus. Fusionprotein; Site-specificrec0mbinati0n: Greenfluorescentprotein mouth agriculture is very business seminars grain consumption of poultry complex and Russia believe l2 15, 2005. Ministry of Agriculture, a major animal disease emergency response command center held a Departments responsible comrades of the Ministry of Agriculture, Chinese Academy of Agricultural Sciences experts and Sinder Technology Group, Jilin Deda,[link widoczny dla zalogowanych], Guangdong Wen's Shandong Zhucheng Foreign Trade, Qingdao Nine Alliance, Shandong, Phoenix and other enterprises attended the seminar. Participants on the current epidemic of highly pathogenic avian influenza affected poultry industry, farming and consumption l to change the way the implementation of policies and measures to support the poultry industry on issues such as in-depth discussion. Meeting that occurred this year, some provinces in China of highly pathogenic bird flu ��Health, poultry products, prices, sales difficulties,[link widoczny dla zalogowanych], inventory, breeding enterprise funds drying up, the market circulation blocked, to the poultry industry in production, processing and related industries with to great losses,[link widoczny dla zalogowanych], but to the present, large-scale farming enterprises and contract farming households did not) students of bird flu, which fully shows that large-scale farming and processing enterprises had 2 years of industrial upgrading, scientific and standardized culture model, the disease Large-scale farming and processing enterprises attach great importance to product. } S ,:',, amount to build a scientific system of prevention and drug residue control, strict management system and security system. Established from source to final product quality of the whole process of product traceability system. They produce safe to eat poultry products, consumers can view was expressed that the upcoming Spring Festival, to provide consumers safe and healthy poultry products, poultry industry is the backbone of China's leading corporate responsibility and obligation to adhere to the leading enterprises in key consumer the principle of supremacy of the interests. Strictly in accordance with national food safety and hygiene standards for poultry farming, processing and management,[link widoczny dla zalogowanych], ensure the production, processing and marketing poultry products safe and healthy, so consumer confidence. Experts suggest that the public want to change consumption patterns, select the brand of products, and consumer science, health spending. Large-scale farming and processing enterprises to develop diverse variety of easy to eat poultry products. Increasing commercialization of poultry products, standard bar codes. So that products can be traced. In the processing areas, r_ ----------------- 1 ------------------ A Bu. A - �� ---------------------------------------- ----- A 61_ ------------------------------------- A - / Xiao qian
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