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PostWysłany: Pon 16:27, 28 Lut 2011    Temat postu: tiffany deutschland 核酸酶&

核酸酶保护试验在黄瓜花叶病毒株系鉴定中的初步应用
vltro…alindependentfuntti~aldomai~forc…andn…in【0Mo11-MicrobeInteract1989;2:91--9613l~pez-GalindezC.∞JA,tiffany deutschland,Me]~mJAalAnalysisofgeneticvariabilityahdmappingofpointmutati~sminfluenm76中国病毒学第11卷virIlshytheRNaseAmismatchcleavagemethodPmcNattAcadSciUSA.1988;85;3522~35264KuruthG.PalukaitisPRNAsequencehererogelleityinnatur~Impulationsthreesate]riteRNAeofcucumber…cviru~Vl,tory burch reva。gY.1989;173:231~240TheApplicationofRibonucleaseProtectionAssayintheIdentificationofStrainsofCucumberMosaicVirusXieXiangmingYuJialinLiuYi(NationalLaboratoryforAgroblotechnologv,Mont Blanc pens,l~jingAgriculturalUni-c~-slty,l~ing100094)KarlHellwaldLeeZhangPeterPalukaitis(DepartmentPlantPathology.CornellUniversity.Ithaca,tory burch shoes,NY14853.USA)TheeDNAsequencesspanningnt1209tont1626ofFny—CMVRNA2(asubgroupIstrainjandm2002tonl24330{Ls-CMVRx(asubgroupIIstrain)V,rereusedf0IprobepreparationThe一labelednegativestrandRNAspreparedbytranscriptioninvitrowerehybridizedwithpu—rKiedRNAsoftWOChineseCMVisolatesfrompepperandtomato.respectivelyTheresultshowsthatthetwo(inesesisolates幽arehbidentitiesofsequencetOtbeFny—CMV,ugg stiefel,thereforetheisolateelongtOtheCMVsubgroupIstrain,Atranscriptionprobecomplementarytothefragmentflankingnt1657tont2125ofK—CMVRN(asubgroupIstrainoriginatedfromChi—najwashybridizedwiththepurifiedRNAsofthetWOChineseCMVisolutesaswel1Therelat~onshipbetweentheI<-CMVstrainandthetwoChineseiso1.ateswascompared.Theapp1.icationofb0nucleaseprotectionassaywasdiscussedjntheidentificationofCMVstrainsKeywordsCucumbermosaicvirus(CMV),Strain,Ribonuclea~protectionaSSay
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