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PostWysłany: Pią 9:15, 14 Sty 2011    Temat postu: ugg stiefel Penicillium chrysogenum, a lack of phy

Penicillium chrysogenum, a lack of physical lifting of glucokinase mutations in glucose metabolism ...


Compared to the mutant parent in mixed units that the normal growth medium. We put D-glucose on the growth in lactose-based medium PNL biosynthetic mutants of Penicillium cable with the lead a marriage rate of strain were compared. Add in the training process) xD - glucose. For comparison it also lists that a grape Jing TD medium level of biosynthesis of penicillin. D_ a glucose to galactose medium,ugg stiefel, biological units into the parent strain rate of penicillin by 50,p90x workout schedule, so dogRz reduced by 20%. D-glucose did not affect the synthesis of dogR. Rent a sudden wild Yi Waste Material cents for the body of B enzyme activity galactose: D a glucose reduction of the parent strain 2 ~ dogR; of: * '9. • Since half of the galactosidase activity. However, half of dogR B-galactosidase had no effect. dogR carbon catabolism seems to rob the regulation changes have taken place, therefore, this strain was further experiments. Blocking the absorption of grapes: an old parental strains showed increased uptake over time,mbt italia, straight up, but dogRs the absorption rate is very low. Glucokinase activity: compared with the parent, dogR a phosphate glucose synthesis of a 6-speed is very slow. Because of the lack of glucokinase dogR Tao, Post cent put it as a glkl, different hexose phosphorylation: To determine whether the other sugar glkl phosphorylation reaction of the extract of Penicillium chrysogenum different hexose monophosphate response. In the parental strains found in the extract has a strong dependence ATp a phosphorylation activity of glucose (glucose kinase). D'2 a FDG in the same extract was also phosphorylated, but the efficiency is very low (only D-glucose, 4.5%) D galactose weak braided acidification, the extract does not appear in the D-fructose phosphorylation activity. glkl lack of D-glucose l glucose phosphorylation activity, only the parent D-glucose phosphorylation 1O. The mutant lacking a D-2-deoxyglucose phosphorylation, but retained the normal phosphorylation of D-galactose activity, but not D-fructose phosphorylation. These results support the conclusion that, glkl lack of D-glucose and D-2 post of a D0G kinase. D ~ glucose-regulated differentiation of penicillin into a one enzyme N: D metabolites on the degradation of a glucose from the culture of the 3s ~ 6O hours of cells in a different penicillin N synthase has a huge negative effect. D parental strains grown in different glucose in the cells of a penicillin N synthetase activity level of a control culture only buried (lactose medium) of l7. However, growth in the same experimental conditions glkl did not demonstrate a difference of penicillin N synthetase activity of a suppression. These results are different in the four consecutive experiments, they explained the experimental degradation of carbon metabolites had no effect on the phenomenon of penicillin production. A glucose 6 ~ APA ~ B has no effect on converting enzyme Show details for concurrence: 6 A APA A acetyl-converting enzyme (Penicillium cable biosynthesis enzymes in the final mixture) from the activity of both parents, but also from the D glkl the impact of a glucose. In the fermentation process, D culture and a glucose enzyme levels were similar in the control culture. Discuss the production of the two yellow mutant of penicillin and glucose on the control of the biosynthesis of penicillin is not the same. One of the many detailed studies of the anti-D-2 mutant of a deoxyglucose (glk1) the lack of glucose kinase activity,UGG España, and on D-glucose uptake rate is very low several microorganisms, including the generation. 6 - lactam strains Streptomycesclavm a erBs,oakley sunglasses, the D-glucose uptake kinase phosphorylation of glucose is very relevant. Phosphorylation seems to spread out the involvement of D-glucose, therefore, the lack of glucose kinase mutant of D-glucose in Hugh transmission capacity has been destroyed. In yeast, a number of anti-D-2 mutations in a deoxyglucose Tiji sugar kinase activity decreased. The same. With two catalytic sites of an unusual sugar, ATP has a kinase in the West of carbon Schwann pig yeast metabolites inhibit the degradation of influence played a role. Experimental results show that the lack of beer yeast hexokinase mutant control. Hexokinase isoenzyme pII change the structure of genes. Resulting in the phosphorylation of glucose and fructose. Huang Qing producing enzyme As-P a 78, there are two ATP hexokinase activity, which may be just the two of beer yeast with sugar kinase has been corresponding. One can make D-glucose phosphorylation in the culture conditions, and the other involved in the phosphorylation of D galactose. glkI mutant lacking D-glucose and D-2-deoxyglucose phosphorylation of a capacity, which shows that these two glucose analogs are to be the same
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